IEEARC

Dr. Manisha Shukla

Healthcare Research Expert | Biotechnology | Immunology | Vaccine Development

Assistant Professor | PhD, Indian Institute of Technology Roorkee

Dr. Manisha Shukla is a biotechnology researcher and Assistant Professor with a PhD from IIT Roorkee. Her expertise spans immunology, infectious diseases, vaccine research, molecular biology, recombinant protein development, and immunodiagnostics. Her scientific contributions include peer-reviewed publications and research presentations at national and international scientific forums. She brings an academic research perspective to biotechnology and science-driven healthcare innovation.

Her work spans infectious disease research, immunological mechanisms, diagnostic applications, and emerging biotechnology approaches. She has contributed to peer-reviewed scientific publications, international research conferences, and collaborative academic activities.

At IEEARC Tech, her research background represents expertise relevant to healthcare innovation, biotechnology research, scientific validation, and the development of science-driven healthcare solutions.

Areas of Expertise

1. Immunology and Infectious Disease Research

• Host immune responses to fungal infections.
• B-cell and antibody-mediated immune protection.
• Systemic candidiasis and non-albicans Candida species.
• Vaccine candidate evaluation and immunological mechanisms.
• Chikungunya virus protein research.

2. Vaccine Development and Immunodiagnostics

• Recombinant vaccine antigen research.
• Recombinant protein expression and purification.
• Evaluation of serological responses.
• Immunodiagnostic assay development and research.
• B-cell and T-cell epitope analysis.
• Immunoinformatics approaches to vaccine research.

3. Molecular Biology and Biotechnology

• Molecular cloning and vector construction.
• Primer design and nucleic acid isolation.
• PCR, RT-PCR, real-time PCR, and RT-LAMP.
• Protein expression, purification, and characterization.
• SDS-PAGE and Western blotting.
• ELISA and immunohistochemistry.
• Flow cytometry and immune-cell analysis.

4. Biomedical and Translational Research

• Laboratory-based biological investigation.
• Experimental evaluation of vaccine candidates.
• Molecular and immunological characterization of pathogens.
• Scientific literature analysis and research interpretation.
• Biotechnology research relevant to diagnostic and therapeutic development.

5. Computational Biology and Research Tools

• Protein–ligand docking.
• Epitope prediction and analysis.
• PyMOL and protein structure visualization.
• Protein database analysis using PDB and UniProt.
• Homology modelling and immunoinformatics workflows.

Academic and Professional Experience

Assistant Professor
Department of Biotechnology
Pandit S. N. Shukla University, Shahdol, Madhya Pradesh
November 2022–Present

• Teaching and mentoring students in biotechnology and related scientific disciplines.
• Conducting and contributing to research activities in biotechnology, immunology, biomedical science, and environmental applications.
• Supervising research activities and supporting scientific inquiry.
• Contributing to academic publications, scientific events, and research collaborations.

Research Associate
Defence Research and Development Establishment (DRDE), Defence Research and Development Organisation (DRDO), Gwalior

The employment dates require verification before publication because the dates in the original CV appear inconsistent.

Education and Qualifications

Qualification	Institution	Year
PhD in Biotechnology-related research	Indian Institute of Technology Roorkee	2021
M.Tech.	Motilal Nehru National Institute of Technology Allahabad	2015
B.E.	Madhav Institute of Technology & Science, Gwalior	2013
Postgraduate Diploma in Personnel Management and Industrial Relations	Annamalai University	2020–2021

Doctoral Research Focus: Analysis of immune responses to candidate vaccine antigens in non-albicans Candida-mediated systemic candidiasis.

Research Areas: B cells and antibodies, infectious diseases, immunology, vaccine development, and immunodiagnostic methods.

Doctoral Supervisor: Dr. Soma Rohatgi, Department of Biosciences and Bioengineering, IIT Roorkee.

Selected Research Publications

Dr. Shukla has contributed to scientific publications addressing infectious diseases, immunology, vaccine research, molecular biotechnology, and biomedical science.

The following publications illustrate her research experience.

Infectious Diseases and Immunology

1. Shukla M. et al. (2020). Vaccination with secreted aspartyl proteinase 2 protein from Candida parapsilosis can enhance survival of mice during Candida tropicalis-mediated systemic candidiasis. Infection and Immunity, 88(10), e00312-20.
2. Shukla M. et al. (2021). The Role of B-Cells and Antibodies against Candida Vaccine Antigens in Invasive Candidiasis. Vaccines, 9(10), 1159.
3. Shukla M. et al. (2021). Expression and purification along with evaluation of serological response and diagnostic potential of recombinant Sap2 protein from C. parapsilosis for use in systemic candidiasis. Journal of Fungi, 7(12), 999.
4. Shukla M. et al. (2022). Expression, Purification, and Refolding of Chikungunya Virus Full-Length Envelope E2 Protein along with B-Cell and T-Cell Epitope Analyses Using Immuno-Informatics Approaches. ACS Omega.
5. Shukla M. et al. (2024). Molecular cloning, expression, and purification, along with in silico epitope analysis of recombinant enolase proteins (a potential vaccine candidate) from Candida albicans and Candida auris. Frontiers in Fungal Biology, 5, 1399546.

Biomedical Research and Public Health

6. Sharma S. K. et al. (2023). Isolation and characterization of emerging Mpox virus from India. Journal of Medical Virology, 95(7), e28911.
7. Ruchi Kumari and Manisha Shukla (2023). Sickle Cell Anaemia: Case Presentations from the Indian Tribal Population and Discussion on the Clinicopathologic Aspects. International Journal of Current Microbiology and Applied Sciences, 12(9), 67–79.
8. Manisha Shukla, Pratishtha Tiwari and Sandeep Purohit (2023). Opportunistic Fungal Urinary Tract Infection: A Case Report. World Journal of Pharmaceutical and Medical Research, 9(10), 267–270.
9. Manisha Shukla and M. A. Ahad (2024). Balancing Act: Navigating the Intricacies of Proteostasis. International Journal of Research in Medical Sciences, 12, 3091–3099.
10. Manisha Shukla, Prakash Narayan and Mahesh Narayan (2023). Role of Matrix Metalloproteinases in Triple Negative Breast Cancer. Book chapter in Proteases in Cancer, Taylor & Francis. Publication status should be confirmed before listing this chapter as published.

Note: The selected publications above are drawn from the supplied CV. Bibliographic details, author order, publication status, DOI information, and journal indexing should be independently verified before the profile goes live. A publication list on the website should not imply that every listed journal is SCI/SCIE-indexed unless that status has been checked.

Awards and Research Recognition

• First Prize Awardee, 40th Madhya Pradesh Young Scientist Congress, Madhya Pradesh Council of Science and Technology (MPCST).
• Qualified the Graduate Aptitude Test in Engineering (GATE), 2013.
• Qualified the Biotechnology Eligibility Test conducted by the Ministry of Science and Technology, Government of India, 2015.
• Received a travel grant from the Ministry of Human Resource Development for presenting research at the American Association of Immunologists meeting in San Diego, USA, in 2019.
• Presented research at international scientific meetings focused on immunology and infectious diseases.
• Delivered scientific lectures on sickle cell anaemia, human genetics, and machine-learning-based diagnosis in paediatric patients with sickle cell anaemia.

Scientific Communication and Academic Engagement

Dr. Shukla has participated in national and international scientific conferences, academic events, research presentations, and scientific outreach initiatives.

Her contributions include participation in the Student Innovation Festival at the India International Science Festival in 2023, scientific presentations at international meetings, and lectures addressing biomedical research and disease-related topics.

These activities reflect her engagement with scientific communication, student development, and interdisciplinary research.

Relevance to Healthcare Innovation

Healthcare innovation requires more than a promising product concept. It also requires scientific understanding, appropriate research methods, evidence generation, and a clear understanding of biological mechanisms.

Dr. Manisha Shukla’s background in biotechnology, immunology, infectious diseases, and molecular research provides a scientific perspective relevant to these challenges.

Her areas of expertise may be particularly relevant to:

• Biotechnology and life-sciences startups.
• Vaccine research and development.
• Infectious disease research.
• Biomedical diagnostics and immunoassay research.
• Molecular biology and recombinant protein development.
• Scientific research collaborations and academic partnerships.
• Early-stage healthcare innovations requiring laboratory research or scientific validation.